Nile Red: A Versatile Fluorescent Compound
Optical Properties and Absorption Spectrum
Nile Red is a fluorescent compound characterized by an excitation peak at 559 nm and an emission peak at 635 nm. When excited at 470 nm, Nile Red exhibits a broad emission spectrum in aqueous solutions. The optical absorption spectrum of Nile Red is significantly affected in cyclodextrins (CDs), indicating the formation of inclusion complexes.
Complex Formation in Cyclodextrins
Spectroscopic evidence suggests the formation of multiple inclusion complexes in both alpha- and beta-cyclodextrins. These complexes alter the optical absorption spectrum of Nile Red, resulting in a broadened and dual shape with an additional blue band in purely aqueous solutions.
Application in Spectroscopic Analysis
The unique optical properties of Nile Red make it a valuable tool in spectroscopic analysis. The Spectrum Viewer web application allows users to interactively view and compare Nile Red spectra, providing insights into its interactions with different environments.
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